菜豆叶片在体内和体外的氨酰基转移核糖核酸合成速率。
Rates of aminoacyl-transfer-ribonucleic acid synthesis in vivo and in vitro by bean leaves.
作者信息
Hall T C, Tao K L
出版信息
Biochem J. 1970 May;117(5):853-9. doi: 10.1042/bj1170853.
Abstract
- A procedure for measuring rates of aminoacyl-tRNA synthesis in vitro and in intact leaves is presented. 2. Leaf discs showed rates close to those of intact leaves. 3. Cell-free preparations showed similar rates when assayed by pyrophosphate exchange, but actual aminoacyl-tRNA formation rates appeared to be much lower. Evidence is presented that dilution of supplied labelled amino acids was a major factor causing the low apparent rates. 4. Attempts to strip endogenous amino acids from plant tRNA resulted in low acceptor capability of the tRNA.
摘要
- 本文介绍了一种在体外和完整叶片中测量氨酰-tRNA合成速率的方法。2. 叶盘显示出与完整叶片相近的速率。3. 无细胞制剂通过焦磷酸交换测定时显示出相似的速率，但实际氨酰-tRNA形成速率似乎要低得多。有证据表明，所供应的标记氨基酸的稀释是导致表观速率低的主要因素。4. 试图从植物tRNA中去除内源性氨基酸导致tRNA的接受能力降低。
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引用本文的文献
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Biochem J. 1971 Feb;121(3):495-501. doi: 10.1042/bj1210495.
本文引用的文献
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
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An enzymic mechanism for amino acid activation in animal tissues.动物组织中氨基酸活化的酶促机制。
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PROTEIN SYNTHESIS BY CELL-FREE EXTRACTS FROM TOBACCO LEAVES. I. AMINO ACID INCORPORATING ACTIVITY OF CHLOROPLASTS IN RELATION TO THEIR STRUCTURE.烟草叶片无细胞提取物的蛋白质合成。I. 叶绿体的氨基酸掺入活性与其结构的关系。
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Interference by sucrose in the estimation of ribonucleic acid by the orcinol method.蔗糖对苔黑酚法测定核糖核酸的干扰。
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