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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Biochem J. 1971 Feb;121(3):495-501. doi: 10.1042/bj1210495.

Factors affecting aminoacyl-tRNA synthesis in vitro by cell-free preparations from bean leaves were investigated. 2. Evidence was obtained that optimum concentrations as well as correct ratios of Mg(2+) and ATP are required for aminoacyl-tRNA synthesis in the bean-leaf system. 3. The results indicated that pH is a controlling factor having differential effects on the formation of individual aminoacyl-tRNA species. The possible micro-regulatory function of pH in protein synthesis in vivo is discussed with special reference to alanyl-tRNA formation. 4. Very low rates of alanine-stimulated pyrophosphate exchange were observed in the absence of tRNA. This observation is discussed relative to proposals about the mechanism of aminoacyl-tRNA synthesis.

对豆叶无细胞制剂体外合成氨酰 - tRNA的影响因素进行了研究。2. 有证据表明，豆叶系统中氨酰 - tRNA合成需要最佳浓度以及Mg(2+)和ATP的正确比例。3. 结果表明，pH是一个控制因素，对单个氨酰 - tRNA种类的形成有不同影响。特别参考丙氨酰 - tRNA的形成，讨论了pH在体内蛋白质合成中可能的微观调节功能。4. 在没有tRNA的情况下，观察到丙氨酸刺激的焦磷酸交换速率非常低。相对于关于氨酰 - tRNA合成机制的提议，对这一观察结果进行了讨论。

Biochem J. 1971 Feb;121(3):495-501. doi: 10.1042/bj1210495.

Factors affecting aminoacyl-tRNA synthesis in vitro by cell-free preparations from bean leaves were investigated. 2. Evidence was obtained that optimum concentrations as well as correct ratios of Mg(2+) and ATP are required for aminoacyl-tRNA synthesis in the bean-leaf system. 3. The results indicated that pH is a controlling factor having differential effects on the formation of individual aminoacyl-tRNA species. The possible micro-regulatory function of pH in protein synthesis in vivo is discussed with special reference to alanyl-tRNA formation. 4. Very low rates of alanine-stimulated pyrophosphate exchange were observed in the absence of tRNA. This observation is discussed relative to proposals about the mechanism of aminoacyl-tRNA synthesis.

对豆叶无细胞制剂体外合成氨酰 - tRNA的影响因素进行了研究。2. 有证据表明，豆叶系统中氨酰 - tRNA合成需要最佳浓度以及Mg(2+)和ATP的正确比例。3. 结果表明，pH是一个控制因素，对单个氨酰 - tRNA种类的形成有不同影响。特别参考丙氨酰 - tRNA的形成，讨论了pH在体内蛋白质合成中可能的微观调节功能。4. 在没有tRNA的情况下，观察到丙氨酸刺激的焦磷酸交换速率非常低。相对于关于氨酰 - tRNA合成机制的提议，对这一观察结果进行了讨论。