Meyer T, Burkart W, Jockusch H
Neurosci Lett. 1979 Jan;11(1):59-62. doi: 10.1016/0304-3940(79)90056-9.
The dependence of the induction of choline acetyltransferase (CAT) on the type of tissue culture, monolayer or piece explants, of spinal cord (SC) and muscle (M) was studied. CAT activities of dissociated SC cells were low but were increased by M monolayers. In piece explants of SC, high CAT activities were achieved independently of muscle cells (chick, mouse). It is therefore likely that in tntact SC tissue factors other than the trophic influence of muscle are able to induce cholinergic maturation. M piece explants on SC monolayers did not induce CAT in, and their outgrowth was inhibited by, SC monolayers.
研究了胆碱乙酰转移酶（CAT）的诱导对脊髓（SC）和肌肉（M）的组织培养类型（单层培养或组织块外植体培养）的依赖性。解离的脊髓细胞的CAT活性较低，但肌肉单层培养可使其活性增加。在脊髓组织块外植体中，无论有无肌肉细胞（鸡、小鼠），都能实现较高的CAT活性。因此，在完整的脊髓组织中，除了肌肉的营养影响外，其他因素可能也能够诱导胆碱能成熟。置于脊髓单层培养物上的肌肉组织块外植体不能诱导脊髓单层培养物中的CAT活性，并且其生长受到脊髓单层培养物的抑制。
