Kim S U, Oh T H, Johnson D D
Neurobiology. 1975 May;5(2):119-27.
Explants of 10-12-day-old embryonic chick spinal cord were cultured for up to 20 days by the "coverslip-roller" method. Morphological development of neurons as shown by the presence of mature neurons, myelinated axons and synaptic structures, was demonstrated by light and electron microscopy. Two important enzymes associated with acetylcholine transmitter metabolism, choline acetyltransferase (ChAc) and acetylcholinesterase (AChE), were assayed in cultures at selected time intervals. The activity of ChAc and AChE exhibited an increase of 60 per cent and 80 per cent, respectively, over a 20-day period. It is concluded that organotypic cultures of embryonic chick spinal cord show differentiation not only in morphological aspects, but also in biochemical terms through progressive development of ChAc and AChE.
采用“盖玻片滚动”法将10 - 12日龄鸡胚脊髓外植体培养长达20天。通过光学显微镜和电子显微镜观察到，成熟神经元、有髓轴突和突触结构的存在表明神经元的形态发育。在选定的时间间隔对培养物中与乙酰胆碱递质代谢相关的两种重要酶，即胆碱乙酰转移酶（ChAc）和乙酰胆碱酯酶（AChE）进行了测定。在20天的时间里，ChAc和AChE的活性分别增加了60%和80%。得出的结论是，鸡胚脊髓的器官型培养不仅在形态学方面表现出分化，而且通过ChAc和AChE的逐步发育在生化方面也表现出分化。
