Bell J L, Atkinson S M, Baron D N
J Clin Pathol. 1970 Sep;23(6):509-13. doi: 10.1136/jcp.23.6.509.
THE GLYCERIDE GLYCEROL ANALYSIS DEPENDS, AFTER SAPONIFICATION OF TRIGLYCERIDES, ON A LINKED ENZYMATIC PROCEDURE USING GLYCEROKINASE, PYRUVATE KINASE, AND LACTATE DEHYDROGENASE: the final conversion of NADH to NAD(+) is followed fluorimetrically. Twenty analyses can be performed per hour on the AutoAnalyzer; recoveries of added triglycerides ranged between 90 and 104%. In a mixed male and female group the normal range for glyceride glycerol was 2.5 to 15.5 mg/100 ml (0.2-1.4 mmol/l) fasting, and 2.5 to 18.0 mg/100 ml (0.2-1.6 mmol/l) postprandially using fresh serum. There was a significant rise postprandially in older men.
甘油三酯皂化后，甘油酯甘油分析依赖于一种联用酶法，该方法使用甘油激酶、丙酮酸激酶和乳酸脱氢酶：通过荧光法监测NADH最终转化为NAD⁺的过程。每小时可在自动分析仪上进行20次分析；添加的甘油三酯回收率在90%至104%之间。在一组男女混合的人群中，空腹时甘油酯甘油的正常范围为2.5至15.5毫克/100毫升（0.2 - 1.4毫摩尔/升），餐后使用新鲜血清时为2.5至18.0毫克/100毫升（0.2 - 1.6毫摩尔/升）。老年男性餐后有显著升高。
