一种用于检测血清或血浆中游离甘油和甘油三酯的半自动酶法测定。

A semi-automated enzymatic assay for free glycerol and triglycerides in serum or plasma.

MacRae A R

Clin Biochem. 1977 Feb;10(1):16-9. doi: 10.1016/s0009-9120(77)90232-6.

Abstract

A relatively inexpensive semi-automated enzymatic procedure is described for the determination of total glycerol with an Auto Analyzer. Free glycerol is determined without saponification in a deproteinized supernatant. 2. The dilution of triglyceride samples during preparation is such that the samples during preparation is such that the samples can be quantified directly from the free glycerol standards by multiplying by 100. 3. A pyruvate kiluent solution is used in the triglyceride sample preparation to increase the precision by improving the accuracy in determining blank optical densities. 4. The end-point reaction is complete in eight minutes, and the assay is linear up to 6 mg% free glycerol or 600 mg% triglycerides. The within-run and between-run coefficients of variation were both 1.5% for the glycerol assay, and 1.0% and 4.3% for the triglyceride assay. The mean recovery of added glycerol was 100.7% and 102.3% for the glycerol and triglyceride assays respectively.

摘要

描述了一种相对廉价的半自动酶法，用于通过自动分析仪测定总甘油。在脱蛋白上清液中不进行皂化反应来测定游离甘油。2. 甘油三酯样品在制备过程中的稀释方式使得制备过程中的样品可以通过乘以100直接从游离甘油标准品中进行定量。3. 在甘油三酯样品制备中使用丙酮酸空白溶液，通过提高空白光密度测定的准确性来提高精密度。4. 终点反应在8分钟内完成，该测定法对于高达6mg%的游离甘油或600mg%的甘油三酯呈线性。甘油测定的批内和批间变异系数均为1.5%，甘油三酯测定的批内和批间变异系数分别为1.0%和4.3%。甘油和甘油三酯测定中添加甘油的平均回收率分别为100.7%和102.3%。