Bush K, Mahler H R, Shiner V J
Science. 1971 Apr 30;172(3982):478-80. doi: 10.1126/science.172.3982.478.
Determination of dissociation constants by two different methods yield the following mean values in 20 millimolar phosphate, pH 7.0, 25 degrees C: 0.27 micromolar for reduced nicotinamide adenine dinucleotide (NADH); 0.29 micromolar for NADH with deuterium in the nicotinamide 4-B position (B-NADD); and 0.46 micromolar for NADH with deuterium in the nicotinamide 4-A position (A-NADD). These results indicate that dehydrogenases are capable of recognizing and distinguishing the appropriate hydrogen in the coenzyme already in the initial binding reaction.
通过两种不同方法测定解离常数，在20毫摩尔磷酸盐、pH 7.0、25摄氏度条件下得出以下平均值：还原型烟酰胺腺嘌呤二核苷酸（NADH）为0.27微摩尔；烟酰胺4 - B位含氘的NADH（B - NADD）为0.29微摩尔；烟酰胺4 - A位含氘的NADH（A - NADD）为0.46微摩尔。这些结果表明，脱氢酶在初始结合反应中就能够识别并区分辅酶中合适的氢。
