Rosenthal L J, Shechmeister I L
Appl Microbiol. 1971 Mar;21(3):400-4. doi: 10.1128/am.21.3.400-404.1971.
This paper describes a microprocedure for the tissue culture assay of vesicular stomatitis virus (VSV) and compares the sensitivity of the method with the conventional plaque assay of viral concentration. Microtiter and plaque assay methods were used in titrations, neutralization tests, and thermoinactivation studies with this virus in chick embryo fibroblasts (CEF) and L, HeLa, and PK cell lines. Titration experiments with VSV by the microtiter procedure on preformed monolayers were significantly more sensitive than the plaque assay (P = 0.05). The results of the neutralization tests and thermoinactivation studies also showed greater ability to detect residual virus by the microtiter procedure (P = 0.10). In addition, the microtiter procedure was simpler, less costly, and more rapid than the plaque assay.
本文描述了一种用于水疱性口炎病毒（VSV）组织培养测定的微量程序，并将该方法的灵敏度与病毒浓度的传统空斑测定法进行了比较。微量滴定法和空斑测定法用于该病毒在鸡胚成纤维细胞（CEF）以及L、HeLa和PK细胞系中的滴定、中和试验和热灭活研究。通过微量滴定程序在预先形成的单层细胞上对VSV进行滴定实验，其灵敏度显著高于空斑测定法（P = 0.05）。中和试验和热灭活研究的结果也表明，微量滴定程序检测残留病毒的能力更强（P = 0.10）。此外，微量滴定程序比空斑测定法更简单、成本更低且更快速。
