豚鼠胰腺细胞膜的组成。3. 酶活性。
Composition of cellular membranes in the pancreas of the guinea pig. 3. Enzymatic activities.
作者信息
Meldolesi J, Jamieson J D, Palade G E
出版信息
J Cell Biol. 1971 Apr;49(1):150-8. doi: 10.1083/jcb.49.1.150.
Abstract
A COMPARATIVE STUDY OF THE ENZYMIC ACTIVITIES OF MEMBRANE FRACTIONS DERIVED FROM GUINEA PIG PANCREATIC HOMOGENATES HAS YIELDED THE FOLLOWING RESULTS
Rough microsomal membranes (derived from the rough ER) have the reductase activities of the two microsomal electron transport systems but lack enzyme activities of Golgi-type (TPPase) and plasmalemmal-type (5'-nucleotidase, beta-leucyl naphthylamidase, Mg-ATPase). Smooth microsomal membranes (derived primarily from the Golgi complex), zymogen granule membranes, and plasmalemmal fractions possess overlapping enzyme activities of plasmalemmal type, in different relative concentrations for each fraction. In addition, the smooth microsomal membranes exhibit TPPase and ADPase activity and share with rough microsomes the reductase activities of the two electron transport chains. Taken together with recent data on the lipid composition of the same fractions (2), these results indicate that the membranes of the pancreatic exocrine cell are chemically and functionally distinct, and hence do not mix with one another during the transport of secretory products.
摘要
对豚鼠胰腺匀浆来源的膜组分的酶活性进行的一项比较研究得出了以下结果
粗糙微粒体膜（源自糙面内质网）具有两种微粒体电子传递系统的还原酶活性，但缺乏高尔基体类型（TPP酶）和质膜类型（5'-核苷酸酶、β-亮氨酰萘基酰胺酶、Mg-ATP酶）的酶活性。光滑微粒体膜（主要源自高尔基体复合体）、酶原颗粒膜和质膜组分具有质膜类型的重叠酶活性，各组分的相对浓度不同。此外，光滑微粒体膜表现出TPP酶和ADP酶活性，并与粗糙微粒体共享两条电子传递链的还原酶活性。结合同一组分脂质组成的最新数据（2），这些结果表明胰腺外分泌细胞膜在化学和功能上是不同的，因此在分泌产物运输过程中不会相互混合。
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本文引用的文献
1
Phosphatases of liver. I. Glucose-6-phosphatase.肝脏磷酸酶。I. 葡萄糖-6-磷酸酶。
J Biol Chem. 1950 Jun;184(2):647-59.
2
A microspectrophotometric method for the determination of cytochrome oxidase.一种测定细胞色素氧化酶的显微分光光度法。
J Biol Chem. 1951 Apr;189(2):665-70.
3
4
5
STUDIES ON PLASMA MEMBRANES. I. CHEMICAL COMPOSITION AND ENZYME CONTENT OF PLASMA MEMBRANES ISOLATED FROM RAT LIVER.细胞膜研究。I. 从大鼠肝脏分离的细胞膜的化学组成和酶含量
Biochim Biophys Acta. 1964 Jul 15;90:126-45. doi: 10.1016/0304-4165(64)90125-4.
6
THE LOCALIZATION OF PHOSPHATASE ACTIVITIES AT THE LEVEL OF ULTRASTRUCTURE.超微结构水平上磷酸酶活性的定位
J Histochem Cytochem. 1964 Feb;12:72-95. doi: 10.1177/12.2.72.
7
Microsomal triphosphopyridine nucleotide-cytochrome c reductase of liver.肝脏微粒体三磷酸吡啶核苷酸-细胞色素c还原酶
J Biol Chem. 1962 Feb;237:587-95.
8
A rapid spectrophotometric assay of mono-amine oxidase based on the rate of disappearance of kynuramine.一种基于犬尿胺消失速率的单胺氧化酶快速分光光度测定法。
J Biol Chem. 1960 Apr;235:1160-3.
9
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Biochim Biophys Acta. 1960 Jan 29;37:570-1. doi: 10.1016/0006-3002(60)90532-1.
10
Purification of acid phosphomonoesterase from the human prostate gland.从人前列腺中纯化酸性磷酸单酯酶。
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