Orci L, Montesano R, Meda P, Malaisse-Lagae F, Brown D, Perrelet A, Vassalli P
Proc Natl Acad Sci U S A. 1981 Jan;78(1):293-7. doi: 10.1073/pnas.78.1.293.
The Golgi apparatus is a key element in the ordered movement of secretory polypeptides from the rough endoplasmic reticulum to the plasma membrane during secretion. It has been shown that cisternae that receive membranes from the reticulum are morphologically similar to the latter and that cisternae liberating secretory granules resemble that plasma membrane. By using an ultrastructural probe for membrane cholesterol, filipin, on freeze-fractured and thin-sectioned exocrine and endocrine pancreatic cells, we have shown that an enrichment in filipin-cholesterol complexes takes places across the stacked cisternae of the Golgi apparatus; the reticulum-related (forming) cisternae are poor in such complexes, but the secretory granule-related (maturing) cisternae contain numerous complexes. Secretory granule membrane is also richly labeled with filipin-cholesterol complexes. The heterogeneous cholesterol distribution in the membranes of the Golgi apparatus, as shown by filipin, emphasizes the polarity of this organelle, in agreement with its role in organizing the traffic of the secretory polypeptides from the rough endoplasmic reticulum to the plasma membrane.
高尔基体是分泌过程中分泌性多肽从粗面内质网有序运输到质膜的关键元件。研究表明，从内质网接收膜的扁平囊在形态上与内质网相似，而释放分泌颗粒的扁平囊则类似于质膜。通过使用膜胆固醇的超微结构探针——制霉菌素，对冷冻断裂和超薄切片的胰腺外分泌细胞和内分泌细胞进行研究，我们发现制霉菌素 - 胆固醇复合物在高尔基体的堆叠扁平囊中富集；与内质网相关的（形成中的）扁平囊中此类复合物较少，但与分泌颗粒相关的（成熟中的）扁平囊中含有大量复合物。分泌颗粒膜也被制霉菌素 - 胆固醇复合物大量标记。制霉菌素显示的高尔基体膜中胆固醇的异质分布强调了该细胞器的极性，这与其在组织分泌性多肽从粗面内质网到质膜的运输中的作用一致。
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