一种将蛋白质与不溶性多糖偶联的简单方法。
A simple method for coupling proteins to insoluble polysaccharides.
作者信息
Sanderson C J, Wilson D V
出版信息
Immunology. 1971 Jun;20(6):1061-5.
Abstract
Proteins have been coupled to insoluble polysaccharides following periodate oxidation to produce reactive aldehyde groups which will react with side chain amino groups of proteins. The coupling is stabilized and unreacted aldehydes blocked, by reduction with sodium borohydride.
摘要
蛋白质在高碘酸盐氧化后与不溶性多糖偶联，以产生能与蛋白质侧链氨基反应的活性醛基。通过用硼氢化钠还原，使偶联稳定，并封闭未反应的醛基。
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本文引用的文献
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The "dialdehydes" from the periodate oxidation of carbohydrates.碳水化合物经高碘酸盐氧化产生的“二醛”
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Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides.通过卤化氰将肽和蛋白质与多糖进行化学偶联。
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