Reiss U, Gershon D
Eur J Biochem. 1976 Apr 1;63(2):617-23. doi: 10.1111/j.1432-1033.1976.tb10266.x.
Cytoplasmic superoxide dismutase has been purified from livers of young (6 months) and old (27 months) rats. The enzyme purified from old animals shows an age-related reduction in the specific activity, accumulation of antigenically cross-reacting material and increased sensitivity to temperature. No differences were found in the molecular weight, electrophoretic mobility, antigenicity and Ki between enzymes purified from young and old rats. This is the first demonstration of age-related alterations in a purified form of a non-metabolic enzyme, which can be related to reduced activity. The possible role of this reduced activity in age-dependent deterioration of cellular functions is discussed.
已从幼年（6个月）和老年（27个月）大鼠的肝脏中纯化出细胞质超氧化物歧化酶。从老年动物中纯化出的这种酶显示出比活性随年龄增长而降低、抗原交叉反应物质的积累以及对温度的敏感性增加。从幼年和老年大鼠中纯化出的酶在分子量、电泳迁移率、抗原性和抑制常数方面未发现差异。这是首次证明一种纯化形式的非代谢酶存在与年龄相关的改变，这种改变可能与活性降低有关。文中讨论了这种活性降低在细胞功能随年龄衰退过程中可能发挥的作用。
