Schmidtke J R, Hatfield S M, Ferguson R M
Transplantation. 1979 May;27(5):319-23. doi: 10.1097/00007890-197905000-00006.
The inhibition of the mitogenic activation of human peripheral blood lymphocyte (PBL) subpopulations by methylprednisolone (MP) was dependent on the mitogen used. Purified human T cells were more sensitive to the effects of MP than were B cells or PBLs, especially when these cells were activated by pokeweed mitogen (PWM). MP did not function by inhibiting binding of mitogen to the cell surface. After being mitogenically activated, human lymphocytes were resistant to the effects of MP. These effects of MP were shown to be reversible. Monocytes did not provide a significant degree of protection to mitogenically activated human T cells incubated with MP. These data suggest that MP-induced inhibition of the mitogenic activation of human PBLs may be a reflection of lymphocyte heterogeneity, and that the differential sensitivity of PBLs to MP may be used to isolate functionally different subpopulations of these cells.
甲基泼尼松龙（MP）对人外周血淋巴细胞（PBL）亚群促有丝分裂激活的抑制作用取决于所使用的有丝分裂原。纯化的人T细胞比B细胞或PBL对MP的作用更敏感，尤其是当这些细胞被商陆有丝分裂原（PWM）激活时。MP并非通过抑制有丝分裂原与细胞表面的结合来发挥作用。人淋巴细胞在被有丝分裂原激活后，对MP的作用具有抗性。MP的这些作用被证明是可逆的。单核细胞对与MP一起孵育的经有丝分裂原激活的人T细胞没有提供显著程度的保护。这些数据表明，MP诱导的对人PBL有丝分裂激活的抑制可能反映了淋巴细胞的异质性，并且PBL对MP的不同敏感性可用于分离这些细胞功能不同的亚群。
