Smith J A, Francis T I, Edington G M, Williams A O
Br J Cancer. 1971 Jun;25(2):343-9. doi: 10.1038/bjc.1971.44.
The indirect immunofluorescence technique demonstrated that human alpha fetoprotein (AFP) was present in a focal pattern in the cytoplasm of malignant liver cells of patients whose sera contained AFP. A few fibroblastlike cells in tissue culture of liver biopsies from patients with hepato-cellular carcinoma and AFP in their sera also had the protein. The intracellular localisation of human AFP was confirmed by centrifugation of washed, homogenised and ultra-sonically disrupted neonatal liver cells. Examination of livers of fetuses and neonates showed that AFP was present predominantly in the periportal parenchymal cells.
间接免疫荧光技术表明，在血清中含有甲胎蛋白（AFP）的患者的恶性肝细胞胞质中，人甲胎蛋白呈局灶性分布。来自肝细胞癌患者且血清中含有AFP的肝脏活检组织培养中的一些成纤维细胞样细胞也含有该蛋白。通过对洗涤、匀浆并经超声破碎的新生肝细胞进行离心，证实了人AFP的细胞内定位。对胎儿和新生儿肝脏的检查表明，AFP主要存在于门静脉周围实质细胞中。
