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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Mendelsohn F A, Johnston C I

Biochem J. 1971 Jan;121(2):241-4. doi: 10.1042/bj1210241.

A synthetic 3-([(14)C]valine)-labelled tetradecapeptide renin substrate was used to measure renin concentration. Renin liberated (14)C-labelled angiotensin I, which was separated from the labelled substrate by paper chromatography. The conversion of substrate into angiotensin I was quantitated by liquid-scintillation counting of radioactivity. 2. The rate of conversion of the substrate into angiotensin I was shown to be linearly related to renin concentration and time under suitable conditions. Angiotensin generation measured in this system agrees well with that measured by bioassay. 3. It is suggested that the use of a pure substrate offers advantages that include the standardization of current units of renin measurement.

一种合成的3-([(14)C]缬氨酸)标记的十四肽肾素底物被用于测量肾素浓度。肾素释放出(14)C标记的血管紧张素I，通过纸层析将其与标记的底物分离。底物向血管紧张素I的转化通过放射性的液体闪烁计数进行定量。2. 在合适的条件下，底物向血管紧张素I的转化速率显示与肾素浓度和时间呈线性关系。在该系统中测量的血管紧张素生成与通过生物测定法测量的结果非常吻合。3. 有人提出，使用纯底物具有多种优势，包括使当前肾素测量单位标准化。

Mendelsohn F A, Johnston C I

Biochem J. 1971 Jan;121(2):241-4. doi: 10.1042/bj1210241.

A synthetic 3-([(14)C]valine)-labelled tetradecapeptide renin substrate was used to measure renin concentration. Renin liberated (14)C-labelled angiotensin I, which was separated from the labelled substrate by paper chromatography. The conversion of substrate into angiotensin I was quantitated by liquid-scintillation counting of radioactivity. 2. The rate of conversion of the substrate into angiotensin I was shown to be linearly related to renin concentration and time under suitable conditions. Angiotensin generation measured in this system agrees well with that measured by bioassay. 3. It is suggested that the use of a pure substrate offers advantages that include the standardization of current units of renin measurement.

一种合成的3-([(14)C]缬氨酸)标记的十四肽肾素底物被用于测量肾素浓度。肾素释放出(14)C标记的血管紧张素I，通过纸层析将其与标记的底物分离。底物向血管紧张素I的转化通过放射性的液体闪烁计数进行定量。2. 在合适的条件下，底物向血管紧张素I的转化速率显示与肾素浓度和时间呈线性关系。在该系统中测量的血管紧张素生成与通过生物测定法测量的结果非常吻合。3. 有人提出，使用纯底物具有多种优势，包括使当前肾素测量单位标准化。