肌原纤维的调节蛋白。抑制因子（肌钙蛋白B）的特性与性质
The regulatory proteins of the myofibril. Characterization and properties of the inhibitory factor (troponin B).
作者信息
Schaub M C, Perry S V
出版信息
Biochem J. 1971 Jul;123(3):367-77. doi: 10.1042/bj1230367.
Abstract
- Gel-filtration results indicate that the major component of inhibitory-factor preparations isolated by dissociation of the troponin complex consisted of a protein of subunit weight 23000 daltons. By the same procedure a molecular weight of 18000 was obtained for the calcium-sensitizing factor. 2. The inhibitory factor is specific for the actomyosin type of ATPase and ITPase. It is effective on desensitized actomyosin, natural actomyosin and intact myofibrils. 3. For inhibition, the actomyosin ATPase must be stimulated by Mg(2+), Ca(2+) or Mn(2+). The Co(2+)-, Cd(2+)- or Zn(2+)-stimulated ATPases are not affected. 4. Biological activity is stable to treatment with dissociating agents, heat, pH11, pH1 and carboxymethylation. 5. Increasing amounts of actin, but not myosin or tropomyosin, progressively neutralize the inhibitory activity when added to desensitized actomyosin or myofibrils.
摘要
- 凝胶过滤结果表明，通过肌钙蛋白复合物解离分离得到的抑制因子制剂的主要成分是一种亚基分子量为23000道尔顿的蛋白质。通过相同的方法，得到钙敏化因子的分子量为18000。2. 抑制因子对肌动球蛋白类型的ATP酶和ITP酶具有特异性。它对脱敏肌动球蛋白、天然肌动球蛋白和完整肌原纤维有效。3. 对于抑制作用，肌动球蛋白ATP酶必须由Mg(2+)、Ca(2+)或Mn(2+)刺激。Co(2+)、Cd(2+)或Zn(2+)刺激的ATP酶不受影响。4. 生物活性对解离剂处理、加热、pH11、pH1和羧甲基化稳定。5. 当添加到脱敏肌动球蛋白或肌原纤维中时，肌动蛋白量的增加会逐渐中和抑制活性，但肌球蛋白或原肌球蛋白则不会。
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