张力间歇性变化对离体兔肌肉中蛋白质和胶原蛋白合成的影响。
The effect of intermittent changes in tension on protein and collagen synthesis in isolated rabbit muscles.
作者信息
Palmer R M, Reeds P J, Lobley G E, Smith R H
出版信息
Biochem J. 1981 Sep 15;198(3):491-8. doi: 10.1042/bj1980491.
Isolated intact rabbit muscles were incubated in a medium containing radioactive proline. The rates of synthesis of collagen and total muscle protein after incubation with a constant tension or intermittent mechanical stretching were compared with the rates in vivo. Muscles incubated under a constant tension synthesized protein at 22% of the rate observed in vivo; intermittent mechanical stretching resulted in an increase of 73% in the rate of protein synthesis, to 38% of that found in vivo. Collagen synthesis was affected in the same way as total protein synthesis by both types of incubation, therefore the relative rates of collagen and total protein synthesis were unchanged. ATP concentration in the isolate muscles and the uptake of glucose from the medium were increased by intermittent mechanical stretching. Incubating the muscles with a gas phase containing 5% O2 decreased the rate of protein synthesis, abolished the effect of intermittent mechanical stretching, lowered the concentration of ATP and increased the lactate concentration. The rate of protein synthesis in muscles maintained with a constant or intermittently applied tension was not affected by a previous period of incubation with the other type of stimulus.
将分离出的完整兔肌肉置于含有放射性脯氨酸的培养基中培养。将在恒定张力或间歇性机械拉伸下培养后的胶原蛋白和总肌肉蛋白的合成速率与体内的合成速率进行比较。在恒定张力下培养的肌肉，其蛋白质合成速率为体内观察到速率的22%；间歇性机械拉伸使蛋白质合成速率提高了73%，达到体内发现速率的38%。两种培养方式下，胶原蛋白合成与总蛋白质合成受到的影响相同，因此胶原蛋白与总蛋白质合成的相对速率未发生变化。间歇性机械拉伸可提高分离肌肉中的ATP浓度以及培养基中葡萄糖的摄取量。将肌肉置于含5% O₂的气相中培养会降低蛋白质合成速率，消除间歇性机械拉伸的作用，降低ATP浓度并增加乳酸浓度。先前用另一种刺激方式培养一段时间后，以恒定或间歇性施加张力维持的肌肉中的蛋白质合成速率不受影响。
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