Rogers S
J Exp Med. 1971 Dec 1;134(6):1442-52. doi: 10.1084/jem.134.6.1442.
The change in the state of the virus-induced enzyme associated with a mutation in the virus provides additional evidence that the enzyme is synthesized from virus rather than rabbit genetic information. This change in structure results in differences in stability of polymerization, degree of optical rotary dispersion (ORD) specific rotation, change in elution characteristics from carboxymethyl cellulose, and a reduction in specific activity of the arginase. Liver arginase differs markedly in ORD characteristics from the virus-induced enzyme. In contrast to the virus-induced enzyme, it showed no negative Cotton effect at 233 nm until it was activated with manganese. Manganese had no influence on the ORD spectrum of virus-induced arginase. In addition, liver arginase is denatured by 4 M urea, while the virus-induced enzyme requires 10 M urea for denaturation.
与病毒突变相关的病毒诱导酶状态的变化提供了额外证据，表明该酶是由病毒而非兔的遗传信息合成的。这种结构变化导致聚合稳定性、旋光色散（ORD）比旋度、羧甲基纤维素洗脱特性的差异以及精氨酸酶比活性的降低。肝脏精氨酸酶的ORD特性与病毒诱导酶明显不同。与病毒诱导酶相反，在未用锰激活之前，它在233nm处没有负科顿效应。锰对病毒诱导的精氨酸酶的ORD光谱没有影响。此外，肝脏精氨酸酶在4M尿素中变性，而病毒诱导酶需要10M尿素才能变性。
