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感染新城疫病毒或脑心肌炎病毒或用嘌呤霉素处理的HeLa细胞脱氧核糖核酸的修复复制

Repair replication of HeLa cell deoxyribonucleic acid in cells infected with Newcastle disease virus or mengovirus or treated with puromycin.

作者信息

Hand R, Tamm I

出版信息

J Virol. 1971 Oct;8(4):597-600. doi: 10.1128/JVI.8.4.597-600.1971.

Abstract

We examined repair replication of HeLa cell deoxyribonucleic acid (DNA) in cells infected with mengovirus or Newcastle disease virus or treated with puromycin. Cellular DNA was damaged by ultraviolet light and then pulse-labeled with (3)H-thymidine. Autoradiographic analysis of non-S-phase DNA synthesis (repair replication) showed that there was no inhibition of this process at a time when overall cellular DNA synthesis was severely inhibited by either virus infection or puromycin treatment.

摘要

我们检测了感染脑心肌炎病毒或新城疫病毒或用嘌呤霉素处理的海拉细胞脱氧核糖核酸(DNA)的修复复制情况。细胞DNA经紫外线损伤,然后用³H-胸腺嘧啶进行脉冲标记。对非S期DNA合成(修复复制)的放射自显影分析表明,在病毒感染或嘌呤霉素处理严重抑制总体细胞DNA合成的同时,这一过程并未受到抑制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c7c/376235/76fb9df8da34/jvirol00286-0245-a.jpg

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