Bader J P, Bader A V
Proc Natl Acad Sci U S A. 1970 Oct;67(2):843-50. doi: 10.1073/pnas.67.2.843.
The requirement for DNA synthesis in the reproduction of RNA tumor viruses was examined using 5-bromodeoxyuridine (BrdU). Addition of BrdU for the first 12 hr after exposure of cells to Rous sarcoma virus resulted in a large decrease in infectious progeny. However, incorporation of [(3)H]uridine into the viral RNA of virions was unaffected. Also, the numbers of virions visualized by electron microscopy were similar in BrdU-treated and untreated samples. Viral group-specific antigen was synthesized after BrdU treatment, and BrdU had little effect on transformation of cells by Rous sarcoma virus. The results support the hypothesis that the replicative genome of RNA-containing tumor viruses contains DNA.
利用5-溴脱氧尿苷（BrdU）研究了RNA肿瘤病毒复制过程中DNA合成的需求。在细胞暴露于劳氏肉瘤病毒后的最初12小时内添加BrdU，导致感染性子代大量减少。然而，[³H]尿苷掺入病毒粒子的病毒RNA中不受影响。此外，通过电子显微镜观察到的病毒粒子数量在BrdU处理组和未处理组中相似。BrdU处理后合成了病毒群特异性抗原，并且BrdU对劳氏肉瘤病毒转化细胞的影响很小。这些结果支持了含RNA肿瘤病毒的复制基因组包含DNA这一假说。
