Kim H U, Goepfert J M
Appl Microbiol. 1971 Oct;22(4):581-7. doi: 10.1128/am.22.4.581-587.1971.
An egg yolk-polymyxin medium (KG) for rapid enumeration of Bacillus cereus is described. The test is presumptive in that differentiation of B. cereus (and closely related organisms) from other species is based on the formation of turbidity in the agar surrounding the colonies of the cereus group organisms. The medium is formulated to encourage sporulation and release of free spores for serological confirmatory tests within the 24-hr incubation period. The production of turbidity in egg yolk and free-spore production by 25 strains of B. cereus on KG agar were measured. The recovery of food poisoning strains of B. cereus inoculated into nonsterile food slurries was assessed. A comparison of KG agar and mannitol-egg yolk-polymyxin-agar indicated that the two media were comparable in their abilities to recover low levels of B. cereus from naturally contaminated foods. Since KG agar enhances spore formation by B. cereus, thus permitting early serological testing, its use in screening food products is advocated.
本文描述了一种用于快速计数蜡样芽孢杆菌的蛋黄 - 多粘菌素培养基（KG）。该检测方法具有推测性，因为蜡样芽孢杆菌（及密切相关的微生物）与其他物种的区分是基于蜡样芽孢杆菌群微生物菌落周围琼脂中浑浊现象的形成。该培养基的配方旨在促进芽孢形成，并在24小时培养期内释放游离芽孢以进行血清学确证试验。测定了25株蜡样芽孢杆菌在KG琼脂上产生蛋黄浑浊和游离芽孢的情况。评估了接种到非无菌食品浆中的蜡样芽孢杆菌食物中毒菌株的回收率。KG琼脂和甘露醇 - 蛋黄 - 多粘菌素琼脂的比较表明，两种培养基从天然污染食品中回收低水平蜡样芽孢杆菌的能力相当。由于KG琼脂可增强蜡样芽孢杆菌的芽孢形成，从而允许早期进行血清学检测，因此提倡将其用于食品产品的筛选。
