一种用于鉴定食品中蜡样芽孢杆菌的荧光抗体程序的功效。
Efficacy of a fluorescent-antibody procedure for identifying Bacillus cereus in foods.
作者信息
Kim H U, Goepfert J M
出版信息
Appl Microbiol. 1972 Nov;24(5):708-13. doi: 10.1128/am.24.5.708-713.1972.
One hundred and seventeen strains of Bacillus were examined by the fluorescent-antibody technique by using the globulin fraction of serum prepared against spores of B. cereus T. All but one strain of the 59 B. cereus tested fluoresced at the exosporium surface. Fluorescent staining of B. anthracis, B. thuringiensis, and B. mycoides was also observed. Absorption of the globulin fraction with B. anthracis and B. mycoides resulted in the elimination of staining of these organisms. Absorption with B. thuringiensis ATCC 10792 removed antibodies reacting with 6 of the strains of B. thuringiensis tested. Absorption with B. thuringiensis var. galleriae removed antibodies against B. cereus to such a degree that the globulin fraction was unusable.
利用针对蜡状芽孢杆菌T型芽孢制备的血清球蛋白组分，通过荧光抗体技术检测了117株芽孢杆菌。在检测的59株蜡状芽孢杆菌中，除1株外，其余菌株在芽孢外壁表面均发出荧光。还观察到炭疽芽孢杆菌、苏云金芽孢杆菌和蕈状芽孢杆菌的荧光染色。用炭疽芽孢杆菌和蕈状芽孢杆菌吸收球蛋白组分后，消除了这些菌的染色。用苏云金芽孢杆菌ATCC 10792吸收后，去除了与所检测的6株苏云金芽孢杆菌反应的抗体。用苏云金芽孢杆菌蜡螟变种吸收后，针对蜡状芽孢杆菌的抗体被去除到球蛋白组分无法使用的程度。
Zotero 插件