Boarer C D, Read W C, Read J J
Appl Microbiol. 1971 Nov;22(5):763-8. doi: 10.1128/am.22.5.763-768.1971.
A highly significant correlation coefficient (r = 0.97, n = 18) was found between the concentration of lactate dehydrogenase measurable after the organisms had been disrupted and the concentration of colony-forming units during the logarithmic phase of growth of a broth culture of the T(1) strain of Mycoplasma mycoides var. mycoides. A concentration of 4.60 x 10(-7) milliunits of lactate dehydrogenase for each colony-forming unit was established. This relationship was used to convert the concentration of lactate dehydrogenase in the culture into an estimate of the concentration of viable mycoplasma. The lactate dehydrogenase was estimated by following the oxidation of reduced nicotinamide adenine dinucleotide, in the presence of pyruvate substrate, at 366 nm in a spectrophotometer. The nicotinamide adenine dinucleotide oxidase system probably contributed a small amount of enzyme activity to the test when lactate dehydrogenase was measured in this way. The method has been described and evaluated for the estimation of titers from 10(7) to 5 x 10(9) colony-forming units per ml.
在丝状支原体丝状亚种T(1)菌株的肉汤培养物对数生长期，发现菌体裂解后可测得的乳酸脱氢酶浓度与菌落形成单位浓度之间存在高度显著的相关系数（r = 0.97，n = 18）。确定每个菌落形成单位的乳酸脱氢酶浓度为4.60×10⁻⁷毫单位。利用这种关系将培养物中乳酸脱氢酶的浓度换算成活支原体浓度的估计值。在分光光度计中于366 nm波长下，在丙酮酸底物存在的情况下，通过跟踪还原型烟酰胺腺嘌呤二核苷酸的氧化来估计乳酸脱氢酶。当以这种方式测量乳酸脱氢酶时，烟酰胺腺嘌呤二核苷酸氧化酶系统可能对测试贡献了少量酶活性。该方法已被描述和评估，用于估计每毫升10⁷至5×10⁹个菌落形成单位的滴度。
