Read W C, Boarer C D, Read J J
Appl Microbiol. 1971 Nov;22(5):769-71. doi: 10.1128/am.22.5.769-771.1971.
The measurement of lactate dehydrogenase activity has been used to evaluate the growth of the T(1) vaccine strain of Mycoplasma mycoides var. mycoides in broth during the production of vaccine. Estimations were made during incubation at 37 C and storage at 4 C. The results were compared with titers of the vaccine obtained by two conventional methods of assessing viability titers: the counting of colonies formed on solid medium and a 50% end point titer found after dilutions were made in broth. The method of measuring lactate dehydrogenase activity allowed the rapid enumeration of organisms during incubation of the culture, but estimations made on vaccine stored at 4 C did not compare well with the two conventional methods of assessing viability. The lactate dehydrogenase activity of the culture has enabled rapid monitoring of the production stages of this vaccine.
乳酸脱氢酶活性的测定已被用于评估在疫苗生产过程中，丝状支原体丝状亚种T(1)疫苗株在肉汤中的生长情况。在37℃培养和4℃储存期间进行了评估。将结果与通过两种传统的评估活力滴度方法获得的疫苗滴度进行了比较：在固体培养基上形成的菌落计数以及在肉汤中稀释后得到的50%终点滴度。测量乳酸脱氢酶活性的方法能够在培养物培养期间快速计数微生物，但对储存在4℃的疫苗进行的评估与两种传统的评估活力方法相比效果不佳。培养物的乳酸脱氢酶活性使得能够快速监测该疫苗的生产阶段。
