Klemme J H, Klemme B, Gest H
J Bacteriol. 1971 Dec;108(3):1122-8. doi: 10.1128/jb.108.3.1122-1128.1971.
Soluble inorganic pyrophosphatases of five species of nonsulfur purple bacteria were investigated in respect to reaction kinetics, regulatory behavior, and other characteristics. The enzymes appear to fall into two groups with correlated properties. The pyrophosphatases of Rhodopseudomonas capsulata and R. spheroides have molecular weights of approximately 60,000, are stabilized by Co(2+), and exhibit simple Michaelis-Menten reaction kinetics. On the other hand, the enzymes of R. palustris, R. gelatinosa, and Rhodospirillum rubrum are larger (molecular weight approximately 100,000), require Zn(2+) for maintenance of catalytic activity, and show complex reaction kinetics; these pyrophosphatases are activated by free Mg(2+) ions and, in the absence of the latter, are inhibited by 2-phosphoglyceric acid. The results described indicate the existence of alternative control patterns for regulation of intracellular turnover of phosphate, which is in part mediated by pyrophosphatases.
对五种非硫紫色细菌的可溶性无机焦磷酸酶的反应动力学、调节行为及其他特性进行了研究。这些酶似乎可分为两组，其特性相互关联。荚膜红假单胞菌和球形红假单胞菌的焦磷酸酶分子量约为60,000，由Co(2+)稳定，并表现出简单的米氏反应动力学。另一方面，沼泽红假单胞菌、胶状红假单胞菌和深红红螺菌的酶更大（分子量约为100,000），需要Zn(2+)来维持催化活性，并表现出复杂的反应动力学；这些焦磷酸酶被游离的Mg(2+)离子激活，在没有Mg(2+)的情况下，会被2-磷酸甘油酸抑制。所述结果表明存在调节细胞内磷酸盐周转的替代控制模式，这部分是由焦磷酸酶介导的。
