Manganiello V, Vaughan M
J Lipid Res. 1972 Jan;13(1):12-6.
In isolated fat cells, the same maximal rate of glycerol production can be induced by epinephrine or ACTH, alone or in combination with each other or with glucagon. With fat cells from rats weighing 150-175 g, the maximal rate of lipolysis attained with glucagon was 75-80% of that produced by epinephrine or ACTH, and with increasing size of the donor rat, the magnitude of the effect of glucagon relative to that of the other hormones declined markedly. In particulate preparations from fat cells of rats weighing 100-125 g, the maximal effect of glucagon on adenyl cyclase activity was about 60% of that of epinephrine, and was significantly less (30%) in preparations from 350-400 g rats. These data are consistent with the hypothesis that with growth of the rat there is a selective decline in the number of glucagon receptors relative to those for epinephrine or ACTH in the fat cell membrane.
在分离的脂肪细胞中，肾上腺素或促肾上腺皮质激素单独使用、相互联合使用或与胰高血糖素联合使用，均可诱导相同的最大甘油生成速率。对于体重150 - 175克大鼠的脂肪细胞，胰高血糖素达到的最大脂解速率是肾上腺素或促肾上腺皮质激素所产生速率的75 - 80%，并且随着供体大鼠体型增大，胰高血糖素相对于其他激素的作用幅度显著下降。在体重100 - 125克大鼠脂肪细胞的微粒体制剂中，胰高血糖素对腺苷酸环化酶活性的最大作用约为肾上腺素的60%，而在体重350 - 400克大鼠的制剂中则显著更低（30%）。这些数据与以下假说一致：随着大鼠生长，脂肪细胞膜中胰高血糖素受体的数量相对于肾上腺素或促肾上腺皮质激素受体选择性减少。
