Gorman R R, Tepperman H M, Tepperman J
J Lipid Res. 1972 Mar;13(2):276-80.
Basal adenyl cyclase activity and its response to epinephrine and glucagon were studied in isolated adipocyte ghosts obtained from fed, starved, refed, and fat-diet-adapted rats. Epinephrine stimulation of adenyl cyclase was significantly increased in fasted rats, but the glucagon response did not change. Rats fasted for 48 hr and refed a high carbohydrate, low fat diet for 48 or 96 hr showed no differences from chow-fed animals in either basal or hormone-stimulated adenyl cyclase activity. Rats adapted to a high fat, low carbohydrate diet showed an initial and transitory increase in basal activity but a progressive loss of epinephrine- and glucagon-stimulated enzyme activities. The loss in hormone responsiveness correlated well with a decrease in hormone-stimulated lipolysis of fat pads and was associated with a significant increase in fat cell diameter.
研究了从喂食、饥饿、再喂食和适应高脂肪饮食的大鼠分离得到的脂肪细胞膜空壳中的基础腺苷酸环化酶活性及其对肾上腺素和胰高血糖素的反应。禁食大鼠中，肾上腺素对腺苷酸环化酶的刺激作用显著增强，但胰高血糖素反应无变化。禁食48小时并再喂食高碳水化合物、低脂肪饮食48或96小时的大鼠，其基础或激素刺激的腺苷酸环化酶活性与喂食普通饲料的动物无差异。适应高脂肪、低碳水化合物饮食的大鼠，基础活性最初出现短暂升高，但肾上腺素和胰高血糖素刺激的酶活性逐渐丧失。激素反应性的丧失与脂肪垫激素刺激的脂解作用降低密切相关，并与脂肪细胞直径显著增加有关。
