Rose W C, Rodey G E, Rimm A A, Truitt R L, Bortin M M
Exp Hematol. 1976 Mar;4(2):90-6.
Treatment of DBA/2 (H-2d) mice with bacterial endotoxin prior to transplantation of their spleen and lymph node cells into immunosuppressed AKR (H-2k) mice prevented acute mortality from graft-versus-host (GVH) disease. AKR mice that received immunocompetent cells from untreated DBA/2 mice had a median survival time (MST) of 13 days. In contrast, AKR mice that received immunocompetent cells from endotoxin-treated DBA/2 donors had an MST of 54 days. Endotoxin treatment of AKR recipients was not essential for preventing mortality from acute GVH disease. Chimerism was proved by demonstrating that the lymphoid cells of long-term surviving AKR mice had the characteristics of DBA/2 lymphoid cells as measured by their response in mixed leukocyte culture (MLC) tests. Spleen cells from endotoxin-treated DBA/2 mice were able to stimulate, and to be stimulated by, AKR spleen cells in MLC assays. Furthermore, spleen cells from endotoxin-treated DBA/2 mice did not suppress the responses of DBA/2 or AKR spleen cells in 'three-party' MLC tests.
在将DBA/2（H-2d）小鼠的脾细胞和淋巴结细胞移植到免疫抑制的AKR（H-2k）小鼠体内之前，先用细菌内毒素处理DBA/2小鼠，可预防移植物抗宿主（GVH）病导致的急性死亡。接受未处理的DBA/2小鼠免疫活性细胞的AKR小鼠的中位生存时间（MST）为13天。相比之下，接受经内毒素处理的DBA/2供体免疫活性细胞的AKR小鼠的MST为54天。对AKR受体进行内毒素处理对于预防急性GVH病导致的死亡并非必不可少。通过证明长期存活的AKR小鼠的淋巴细胞在混合淋巴细胞培养（MLC）试验中的反应所测量到的具有DBA/2淋巴细胞的特征，证实了嵌合体的存在。在MLC试验中，经内毒素处理的DBA/2小鼠的脾细胞能够刺激AKR脾细胞，并被AKR脾细胞刺激。此外，在“三方”MLC试验中，经内毒素处理的DBA/2小鼠的脾细胞不会抑制DBA/2或AKR脾细胞的反应。
