[C-14 serotonin uptake by turkey thrombocytes].

Centrifugation technique of thrombocytes through silicone oil permits rapid separation of cells from plasma with simultaneous measurement of plasma trapped between the cells in the pellet. This technique was used for determination of 5-HT uptake by turkey thrombocytes. The volume of trapped plasma proportional to the number of thrombocytes was measured using 125I-human serum albumin as a marker. The uptake of 14C-serotonin by thrombocytes was measured as the difference between total radioactivity and that of the trapped plasma. It was shown that turkey thrombocytes like mammalian platelets are able to take up great amounts of serotonin.