化学性交感神经切除术对猫脾脏和虹膜中乙酰胆碱、胆碱含量及胆碱乙酰转移酶活性的影响。

Effect of chemical sympathectomy on the content of acetylcholine, choline and choline acetyltransferase activity in the cat spleen and iris.

Consolo S, Garattini S, Ladinsky H, Thoenen H

J Physiol. 1972 Feb;220(3):639-46. doi: 10.1113/jphysiol.1972.sp009727.

Abstract

Acetylcholine and choline were measured in the spleens and irides of normal and 6-hydroxydopamine-treated cats. In addition, choline acetyltransferase activity was measured in the spleens.2. No acetylcholine or choline acetyltransferase activity were found in spleens of normal or treated cats. The choline content of normal spleens was 12.4 +/- 1.5 mug/g wet wt. (mean +/- S.E. of mean), which was not significantly altered by chemical sympathectomy.3. The acetylcholine and choline contents of the cat iris were 3.0 +/- 0.3 mug/g wet wt. and 7.7 +/- 0.9 mug/g wet wt., respectively. There was no difference in acetylcholine and choline concentrations between left and right or normal and sympathectomized irides.4. These results are discussed in relation to the question of a cholinergic link in post-ganglionic sympathetic transmission.

摘要

测定了正常猫和经6-羟基多巴胺处理的猫的脾脏及虹膜中的乙酰胆碱和胆碱。此外，还测定了脾脏中的胆碱乙酰转移酶活性。
在正常猫或经处理的猫的脾脏中未发现乙酰胆碱或胆碱乙酰转移酶活性。正常脾脏的胆碱含量为12.4±1.5微克/克湿重（平均值±平均标准误），化学交感神经切除术后该含量未发生显著变化。
猫虹膜中的乙酰胆碱和胆碱含量分别为3.0±0.3微克/克湿重和7.7±0.9微克/克湿重。左右虹膜之间以及正常虹膜与交感神经切除术后的虹膜之间，乙酰胆碱和胆碱浓度没有差异。
结合节后交感神经传递中胆碱能联系的问题对这些结果进行了讨论。