兔耳动脉交感神经中的胆碱乙酰转移酶活性
Choline acetyltransferase activity in the sympathetic nerves of the rabbit ear artery.
作者信息
Florence V M, Hume W R, Matsunaga M L
出版信息
Br J Pharmacol. 1981 Jul;73(3):721-4. doi: 10.1111/j.1476-5381.1981.tb16807.x.
1 No statistically significant difference in the activity of choline acetyltransferase (ChAT) was detected between sympathetically denervated and control rabbit ear artery (REA) tissue. This was interpreted as evidence against the hypothesis that endogenous acetylcholine plays an obligatory role in sympathetic postganglionic neurotransmission. 2 The values obtained for ChAT activity in the extraneuronal REA tissue were very low, but were greater than the boiled blank values. 3 Treatment with a specific inhibitor of ChAT did not reduce the REA values, while it did for rabbit iris. Addition of acetylcholinesterase to the REA assay reduced the activity of the collectable product to a markedly lesser degree than was observed with other tissues. 4 The specificity of the enzyme assay at the very low yield levels observed in the extraneuronal REA tissue was therefore questioned.
1 在去交感神经支配的兔耳动脉（REA）组织与对照兔耳动脉组织之间，未检测到胆碱乙酰转移酶（ChAT）活性有统计学显著差异。这被解释为反对内源性乙酰胆碱在交感神经节后神经传递中起必需作用这一假说的证据。2 在神经外REA组织中获得的ChAT活性值非常低，但高于煮沸的空白值。3 用ChAT的特异性抑制剂处理并未降低REA的值，而对兔虹膜的值有降低作用。向REA测定中添加乙酰胆碱酯酶，与其他组织相比，可收集产物的活性降低程度明显较小。4 因此，对在神经外REA组织中观察到的极低产量水平下酶测定的特异性提出了质疑。
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