锰离子作为自旋标记物用于线粒体对锰摄取的研究。
Manganous ion as a spin label in studies of mitochondrial uptake of manganese.
作者信息
Gunter T E, Puskin J S
出版信息
Biophys J. 1972 Jun;12(6):625-35. doi: 10.1016/S0006-3495(72)86108-3.
Abstract
Manganous ion (Mn(2+)) has been used as a spin label for studies of divalent cation uptake by rat liver mitochondria. Spin exchange, observed in the electron paramagnetic resonance (EPR) spectrum of a fraction of the transported Mn(2+), shows that this fraction is bound in regions of high local concentration within the mitochondria. The average separation of manganese ions in that fraction is estimated to be 4.0 +/-0.6 A at the time of greatest concentration.
摘要
锰离子（Mn(2+)）已被用作自旋标记物，用于研究大鼠肝脏线粒体对二价阳离子的摄取。在一部分转运的Mn(2+)的电子顺磁共振（EPR）光谱中观察到的自旋交换表明，这部分Mn(2+)结合在线粒体内局部高浓度区域。在浓度最高时，该部分中锰离子的平均间距估计为4.0±0.6埃。
相似文献
1
Manganous ion as a spin label in studies of mitochondrial uptake of manganese.锰离子作为自旋标记物用于线粒体对锰摄取的研究。
Biophys J. 1972 Jun;12(6):625-35. doi: 10.1016/S0006-3495(72)86108-3.
2
Quantitative magnetic resonance studies of manganese uptake by mitochondria.线粒体对锰摄取的定量磁共振研究。
Biophys J. 1975 Apr;15(4):319-33. doi: 10.1016/S0006-3495(75)85822-X.
3
Ion and pH gradients across the transport membrane of mitochondria following Mn ++ uptake in the presence of acetate.在乙酸盐存在的情况下摄取Mn++后，线粒体内跨运输膜的离子和pH梯度。
Biochem Biophys Res Commun. 1973 Apr 2;51(3):797-803. doi: 10.1016/0006-291x(73)91385-5.
4
Magnetic resonance studies on the mitochondrial divalent cation carrier.线粒体二价阳离子载体的磁共振研究。
Biochim Biophys Acta. 1975 Jan 14;375(1):69-86. doi: 10.1016/0005-2736(75)90073-5.
5
ESR determination of Mn++ uptake and binding in mitochondria.通过红细胞沉降率测定线粒体对二价锰离子的摄取和结合情况。
FEBS Lett. 1975 Dec 15;60(2):354-8. doi: 10.1016/0014-5793(75)80748-4.
6
The effect of endogenous phosphate on the H+/Mn2+ ratio and the state of Mn2+ in the mitochondrial matrix.内源性磷酸盐对线粒体基质中H⁺/Mn²⁺ 比值及Mn²⁺ 状态的影响。
Eur J Biochem. 1976 Dec;71(1):93-9. doi: 10.1111/j.1432-1033.1976.tb11093.x.
7
On the role of inorganic phosphate in divalent-cation sequestration by mitochondria.无机磷酸盐在线粒体对二价阳离子螯合作用中的作用
Eur J Biochem. 1980 May;106(2):425-9. doi: 10.1111/j.1432-1033.1980.tb04588.x.
8
The nature of the electron spin resonance signal during aerobic uptake of Mn2+ in mitochondria from rat liver.
Eur J Biochem. 1983 Aug 1;134(2):385-90. doi: 10.1111/j.1432-1033.1983.tb07579.x.
9
The use of electron paramagnetic resonance in studies of free and bound divalent cation: the measurement of membrane potentials in mitochondria.
Ann N Y Acad Sci. 1975 Dec 30;264:112-23. doi: 10.1111/j.1749-6632.1975.tb31478.x.
10
An electron paramagnetic resonance study of Mn2+ uptake by the chick chorioallantoic membrane.鸡胚绒毛尿囊膜对Mn2+摄取的电子顺磁共振研究
Biochim Biophys Acta. 1976 Mar 19;426(3):557-69. doi: 10.1016/0005-2736(76)90399-0.
引用本文的文献
1
The role of redox-active iron, copper, manganese, and redox-inactive zinc in toxicity, oxidative stress, and human diseases.氧化还原活性铁、铜、锰以及氧化还原非活性锌在毒性、氧化应激和人类疾病中的作用。
EXCLI J. 2025 Jul 25;24:880-954. doi: 10.17179/excli2025-8449. eCollection 2025.
2
Mechanisms of Metal-Induced Mitochondrial Dysfunction in Neurological Disorders.金属诱导的神经疾病中线粒体功能障碍的机制
Toxics. 2021 Jun 17;9(6):142. doi: 10.3390/toxics9060142.
3
Mitochondrial Redox Dysfunction and Environmental Exposures.线粒体氧化还原功能障碍与环境暴露
Antioxid Redox Signal. 2015 Aug 20;23(6):578-95. doi: 10.1089/ars.2015.6289. Epub 2015 Apr 29.
4
Genetic factors and manganese-induced neurotoxicity.遗传因素与锰诱导的神经毒性。
Front Genet. 2014 Aug 4;5:265. doi: 10.3389/fgene.2014.00265. eCollection 2014.
5
Manganese in health and disease.健康与疾病中的锰
Met Ions Life Sci. 2013;13:199-227. doi: 10.1007/978-94-007-7500-8_7.
6
Mitochondrial metals as a potential therapeutic target in neurodegeneration.线粒体金属作为神经退行性疾病的潜在治疗靶点。
Br J Pharmacol. 2014 Apr;171(8):2159-73. doi: 10.1111/bph.12513.
7
Manganese neurotoxicity and the role of reactive oxygen species.锰神经毒性及其与活性氧的关系。
Free Radic Biol Med. 2013 Sep;62:65-75. doi: 10.1016/j.freeradbiomed.2013.01.032. Epub 2013 Feb 8.
8
Role of manganese in neurodegenerative diseases.锰在神经退行性疾病中的作用。
J Trace Elem Med Biol. 2011 Dec;25(4):191-203. doi: 10.1016/j.jtemb.2011.08.144. Epub 2011 Oct 1.
9
Characteristics and possible functions of mitochondrial Ca(2+) transport mechanisms.线粒体钙离子转运机制的特征及可能功能
Biochim Biophys Acta. 2009 Nov;1787(11):1291-308. doi: 10.1016/j.bbabio.2008.12.011. Epub 2009 Jan 6.
10
Manganese accumulates primarily in nuclei of cultured brain cells.锰主要在培养的脑细胞的细胞核中积累。
Neurotoxicology. 2008 May;29(3):466-70. doi: 10.1016/j.neuro.2008.02.012. Epub 2008 Mar 6.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Absolute yield measurements of radiation-produced radicals by electron spin resonance.通过电子自旋共振对辐射产生的自由基进行绝对产率测量。
Phys Med Biol. 1962 Apr;6:599-604. doi: 10.1088/0031-9155/6/4/308.
3
[Electron spin resonances in irradiated bacteriophages and their components. I].[辐照噬菌体及其成分中的电子自旋共振。I]
Strahlentherapie. 1963 Feb;120:161-90.
4
[Structure and polymorphism of lipids: x-ray diffraction study of the system formed from beef heart mitochondria lipids and water].[脂质的结构与多态性：牛心线粒体脂质与水形成体系的X射线衍射研究]
J Mol Biol. 1967 Jul 28;27(2):303-22. doi: 10.1016/0022-2836(67)90022-8.
5
High affinity and low affinity binding of Ca++ by rat liver mitochondria.
J Biol Chem. 1969 Feb 25;244(4):584-93.
6
The interaction of La 3+ with mitochondria in relation to respiration-coupled Ca 2+ transport.镧离子（La³⁺）与线粒体在呼吸偶联钙（Ca²⁺）转运方面的相互作用。
Arch Biochem Biophys. 1971 Apr;143(2):506-15. doi: 10.1016/0003-9861(71)90235-9.
7
A mechanism for the reactions of calcium with mitochondria.钙与线粒体反应的一种机制。
Proc Natl Acad Sci U S A. 1965 May;53(5):1069-76. doi: 10.1073/pnas.53.5.1069.
8
Mitochondria and calcium ion transport.线粒体与钙离子运输
Biochem J. 1970 Sep;119(2):129-38. doi: 10.1042/bj1190129.
9
Electron paramagnetic resonance spectra of manganese (II)-protein complexes. Manganese (II)-concanavalin A.
J Biol Chem. 1970 Feb 10;245(3):662-4.
10
The binding of calcium at lipid-water interfaces.钙在脂质-水界面的结合。
Eur J Biochem. 1967 Mar;1(1):61-9. doi: 10.1007/978-3-662-25813-2_11.
Zotero 插件