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锰离子作为自旋标记物用于线粒体对锰摄取的研究。

Manganous ion as a spin label in studies of mitochondrial uptake of manganese.

作者信息

Gunter T E, Puskin J S

出版信息

Biophys J. 1972 Jun;12(6):625-35. doi: 10.1016/S0006-3495(72)86108-3.

Abstract

Manganous ion (Mn(2+)) has been used as a spin label for studies of divalent cation uptake by rat liver mitochondria. Spin exchange, observed in the electron paramagnetic resonance (EPR) spectrum of a fraction of the transported Mn(2+), shows that this fraction is bound in regions of high local concentration within the mitochondria. The average separation of manganese ions in that fraction is estimated to be 4.0 +/-0.6 A at the time of greatest concentration.

摘要

锰离子(Mn(2+))已被用作自旋标记物,用于研究大鼠肝脏线粒体对二价阳离子的摄取。在一部分转运的Mn(2+)的电子顺磁共振(EPR)光谱中观察到的自旋交换表明,这部分Mn(2+)结合在线粒体内局部高浓度区域。在浓度最高时,该部分中锰离子的平均间距估计为4.0±0.6埃。

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