线粒体对锰摄取的定量磁共振研究。
Quantitative magnetic resonance studies of manganese uptake by mitochondria.
作者信息
Gunter R E, Puskin J S, Russell P R
出版信息
Biophys J. 1975 Apr;15(4):319-33. doi: 10.1016/S0006-3495(75)85822-X.
Abstract
The uptake of the paramagnetic ion manganese by rat liver mitochondria is studied by electron paramagnetic resonance (EPR) spectroscopy. Emphasis is placed on: (a) obtaining accurate EPR quantitation of intramitochondrial manganese fractions previously described (Gunter, T. E., and J. S.Puskin, 1972, Biophys. J. 12:625) (b) establishing competition for intramitochondrial binding between one of these fractions and calcium, (c) demonstrating the effects of substrate and ATP concentrations on each fraction observed through EPR, and (d) demonstrating the effect of inorganic phosphate (Pi) concentration and pH on each fraction.
摘要
通过电子顺磁共振（EPR）光谱研究大鼠肝脏线粒体对顺磁性离子锰的摄取。重点在于：（a）对先前描述的线粒体内锰组分进行准确的EPR定量分析（冈特，T.E.，和J.S.普斯金，1972年，《生物物理杂志》12:625）；（b）确定这些组分之一与钙在线粒体内结合的竞争关系；（c）通过EPR证明底物和ATP浓度对每个观察到的组分的影响；以及（d）证明无机磷酸盐（Pi）浓度和pH值对每个组分的影响。
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本文引用的文献
1
THE ENERGY-LINKED REACTION OF CALCIUM WITH MITOCHONDRIA.钙与线粒体的能量偶联反应
J Biol Chem. 1965 Jun;240:2729-48.
2
EFFECT OF ACTIVE ACCUMULATION OF CALCIUM AND PHOSPHATE IONS ON THE STRUCTURE OF RAT LIVER MITOCHONDRIA.钙和磷酸根离子的主动积累对大鼠肝脏线粒体结构的影响
J Cell Biol. 1964 Oct;23(1):21-38. doi: 10.1083/jcb.23.1.21.
3
Respiration-dependent accumulation of inorganic phosphate and Ca ions by rat liver mitochondria.大鼠肝线粒体依赖呼吸作用对无机磷酸盐和钙离子的积累
Biochem Biophys Res Commun. 1963 Mar 25;10:444-8. doi: 10.1016/0006-291x(63)90377-2.
4
Ca ion uptake by rat kidney mitochondria and its dependence on respiration and phosphorylation.大鼠肾线粒体对钙离子的摄取及其对呼吸作用和磷酸化作用的依赖性。
J Biol Chem. 1962 Aug;237:2670-7.
5
A hydrogen ion concentration gradient in a mitochondrial membrane.
Nature. 1966 Oct 22;212(5060):369-72. doi: 10.1038/212369a0.
6
Electron microscope studies on the active accumulation of Sr++ by rat-liver mitochondria.大鼠肝脏线粒体对Sr++主动积累的电子显微镜研究。
J Cell Biol. 1966 Apr;29(1):37-61. doi: 10.1083/jcb.29.1.37.
7
Spectrophotometric measurements of the kinetics of Ca2+ and Mn2+ accumulation in mitochondria.线粒体中Ca2+和Mn2+积累动力学的分光光度测量。
Biochemistry. 1968 Nov;7(11):4059-63. doi: 10.1021/bi00851a038.
8
Energy dependent bivalent cation translocation in rat liver mitochondria.大鼠肝线粒体中能量依赖的二价阳离子转运
Eur J Biochem. 1970 Feb;12(2):387-91. doi: 10.1111/j.1432-1033.1970.tb00863.x.
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Eur J Biochem. 1969 Oct;10(3):484-93. doi: 10.1111/j.1432-1033.1969.tb00715.x.
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