Rudner R, Ledoux M, Mazelis A
Proc Natl Acad Sci U S A. 1972 Sep;69(9):2745-9. doi: 10.1073/pnas.69.9.2745.
The distribution of pyrimidine oligodeoxynucleotide clusters in L and H strands of Bacillus subtilis DNA separated by methylated albumin-Kieselguhr has been determined. Preparations of native and single-stranded DNA were degraded with diphenylamine in formic acid, and the released isostichs with the general formula of Py(n)P(n+1) were separated on DEAE-cellulose by chain length. Eleven isostichs were obtained for strands L and H in unequal proportions. Each isostich fraction was subfractionated by base composition on DEAE-cellulose at pH 3.0. 61 Pyrimidine oligonucleotide clusters were separated from the H strand and only 46 from the L strand. The findings show a higher degree of asymmetry between the strands in the distribution of cytosine-rich clusters as compared with thymine-rich clusters. The longest cytosine oligodeoxynucleotide present in both strands is of chain length 5. There is an unusually high distribution of thymine oligodeoxynucleotides of length 5-11. Up to chain length 6, the distribution of thymine oligodeoxynucleotides between the strands is about equal; from chain length 7 to 11 they occur predominantly in the H strand.
已确定通过甲基化白蛋白 - 硅藻土分离的枯草芽孢杆菌DNA的L链和H链中嘧啶寡脱氧核苷酸簇的分布。天然和单链DNA制剂在甲酸中用二苯胺降解，释放出的通式为Py(n)P(n + 1)的等长片段通过链长在DEAE - 纤维素上分离。L链和H链获得了11个不等比例的等长片段。每个等长片段部分在pH 3.0的DEAE - 纤维素上按碱基组成进行细分。从H链中分离出61个嘧啶寡核苷酸簇，从L链中仅分离出46个。研究结果表明，与富含胸腺嘧啶的簇相比，富含胞嘧啶的簇在链间分布的不对称程度更高。两条链中存在的最长胞嘧啶寡脱氧核苷酸链长为5。长度为5 - 11的胸腺嘧啶寡脱氧核苷酸分布异常高。链长达到6时，两条链之间胸腺嘧啶寡脱氧核苷酸的分布大致相等；链长从7到11时，它们主要出现在H链中。
