Sumida-Yasumoto C, Doi R H
J Bacteriol. 1974 Feb;117(2):775-82. doi: 10.1128/jb.117.2.775-782.1974.
The messenger ribonucleic acid (mRNA) pattern of log-phase and sporulating cells of Bacillus subtilis has been analyzed by deoxyribonucleic acid (DNA)-RNA hybrid studies with the complementary-strand fragments of DNA. Approximately 80% of the mRNA of log-phase and sporulating cells from stages I, III, and IV hybridizes with the heavy DNA fragments, and 20% hybridizes with the light DNA fragments. Hybrid competition studies indicated that there was either a greatly reduced rate of transcription or a turn-off of some log-phase genes during the sporulation stages. However, a significant amount of log-phase gene transcription occurred even at late stages of sporulation. Similar studies indicate a significantly increased rate of transcription or a turn-on of sporulation phase genes during the latter stages of sproulation. There is a sequential increase in the amount of sporulation-specific transcription from both complementary-strand fragments of DNA. These results indicate that the RNA polymerase population in sporulating cells can transcribe both log-phase and sporulation-phase genes.
利用DNA互补链片段通过脱氧核糖核酸（DNA）-核糖核酸（RNA）杂交研究，分析了枯草芽孢杆菌对数期细胞和产孢细胞的信使核糖核酸（mRNA）模式。来自I、III和IV期的对数期细胞和产孢细胞的mRNA中，约80%与重DNA片段杂交，20%与轻DNA片段杂交。杂交竞争研究表明，在产孢阶段，要么转录速率大幅降低，要么一些对数期基因关闭。然而，即使在产孢后期，仍有大量对数期基因转录发生。类似研究表明，在产孢后期，转录速率显著增加或产孢期基因开启。来自DNA两条互补链片段的产孢特异性转录量呈顺序增加。这些结果表明，产孢细胞中的RNA聚合酶群体可以转录对数期基因和产孢期基因。
