感染野生型腺病毒的口咽脱落上皮细胞的病理学

McCormick D P, Galapon Q, Berling C

Appl Microbiol. 1972 Sep;24(3):389-97. doi: 10.1128/am.24.3.389-397.1972.

Adenovirus disease was diagnosed in 2 hr by staining exfoliated infected oropharyngeal cells with fluorescein-tagged rabbit antisera to adenovirus types 4 and 7. The method was as sensitive as the standard virus isolation procedures, but serological cross reactions were observed. Viral antigens were detected in both the nucleus and cytoplasm of infected cells. Infection was accompanied by the outpouring of large numbers of polymorphonuclear leukocytes and smaller numbers of mononuclear cells. The method provides a model for the study of the cellular response to viral upper respiratory disease.

通过用异硫氰酸荧光素标记的兔抗4型和7型腺病毒抗血清对脱落的感染性口咽细胞进行染色，在2小时内诊断出腺病毒疾病。该方法与标准病毒分离程序一样敏感，但观察到血清学交叉反应。在受感染细胞的细胞核和细胞质中均检测到病毒抗原。感染伴随着大量多形核白细胞和少量单核细胞的涌出。该方法为研究细胞对病毒性上呼吸道疾病的反应提供了一个模型。