Davis N L, Rueckert R R
J Virol. 1972 Nov;10(5):1010-20. doi: 10.1128/JVI.10.5.1010-1020.1972.
A ribonucleoprotein particle containing about 20% ribonucleic acid (RNA), and containing little if any phospholipid or glucosamine, was recovered in high yield after treatment of Schmidt-Ruppin strain of Rous sarcoma virus and B77 virus with the nonionic detergent Nonidet P-40. This structure, which probably derives from the internal ribonucleoprotein filament described in electron microscopy studies, contained 80 to 90% of the viral 60 to 70S RNA and only about 10% of the protein present in intact virions. It sedimented in glycerol density gradients at approximately 130S and had a buoyant density in sucrose of about 1.34 g/ml. Studies with (32)P-labeled virus indicated that the ribonucleoprotein particle contained approximately 30 4S RNA molecules per 10(7) daltons of high-molecular-weight viral RNA. Intact virions contained about 70 4S RNA molecules per 10(7) daltons of high-molecular-weight RNA. Electrophoretic studies in dodecyl sulfate-containing polyacrylamide gels showed that the ribonucleoprotein particle contained only 5 of the 11 polypeptides found in the virion; of these the major component was a polypeptide weighing 14,000 daltons.
用非离子去污剂诺乃洗涤剂P - 40处理劳氏肉瘤病毒的施密特 - 鲁平株和B77病毒后,可高产回收一种核糖核蛋白颗粒,该颗粒含有约20%的核糖核酸(RNA),几乎不含磷脂或氨基葡萄糖。这种结构可能源自电子显微镜研究中描述的内部核糖核蛋白丝,它包含病毒60至70S RNA的80%至90%,而完整病毒粒子中仅约10%的蛋白质。它在甘油密度梯度中沉降至约130S,在蔗糖中的浮力密度约为1.34 g/ml。用(32)P标记病毒的研究表明,每10(7)道尔顿的高分子量病毒RNA,核糖核蛋白颗粒约含30个4S RNA分子。每10(7)道尔顿的高分子量RNA,完整病毒粒子约含70个4S RNA分子。在含十二烷基硫酸钠的聚丙烯酰胺凝胶中的电泳研究表明,核糖核蛋白颗粒仅含病毒粒子中发现的11种多肽中的5种;其中主要成分是一种分子量为14,000道尔顿的多肽。