劳氏肉瘤病毒病毒粒子中的酶和核苷酸。
Enzymes and nucleotides in virions of Rous sarcoma virus.
作者信息
Mizutani S, Temin H M
出版信息
J Virol. 1971 Oct;8(4):409-16. doi: 10.1128/JVI.8.4.409-416.1971.
Abstract
In addition to the previously described deoxyribonucleic acid (DNA) polymerase, DNA ligase, DNA exonuclease, and DNA endonuclease activities, purified virions of Schmidt-Ruppin strain of Rous sarcoma virus (SRV) have nucleotides and nucleotide kinase, phosphatase, hexokinase, and lactate dehydrogenase activities. The SRV virions have no glucose-6-phosphate dehydrogenase activity. All enzyme activities, but glucose-6-phosphate dehydrogenase and adenosine triphosphatase, were increased by disruption of the virions. The DNA polymerase, DNA ligase, and hexokinase activities had a higher specific activity in purified virion cores. It is suggested that during assembly virions of SRV may pick up cytoplasmic components which bind to virion proteins. The role of these components in viral replication is not known at present.
摘要
除了先前描述的脱氧核糖核酸（DNA）聚合酶、DNA连接酶、DNA外切核酸酶和DNA内切核酸酶活性外，劳氏肉瘤病毒施密特-鲁平株（SRV）的纯化病毒粒子还具有核苷酸及核苷酸激酶、磷酸酶、己糖激酶和乳酸脱氢酶活性。SRV病毒粒子没有葡萄糖-6-磷酸脱氢酶活性。除葡萄糖-6-磷酸脱氢酶和三磷酸腺苷酶外，所有酶活性在病毒粒子被破坏后均有所增加。DNA聚合酶、DNA连接酶和己糖激酶活性在纯化的病毒粒子核心中具有更高的比活性。有人提出，在组装过程中，SRV病毒粒子可能摄取与病毒粒子蛋白结合的细胞质成分。目前尚不清楚这些成分在病毒复制中的作用。
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