Guerra H, Deter R L, Williams R P
Infect Immun. 1972 Apr;5(4):513-23. doi: 10.1128/iai.5.4.513-523.1972.
The distribution of Brucella melitensis in various tissues and in subcellular fractions obtained from liver was investigated to evaluate the initial phases of brucellosis in the guinea pig. Fifty minutes after intravenous infection, brucellae were found principally in the blood and liver, with a substantial number recovered from spleen. Fractionation of liver established that most bacteria were found in the mitochondrial plus lysosomal (M + L) fraction; a significant number, however, sedimented in the nuclear (N) fraction. With time, there was a progressive shift of bacteria from the M + L to the N fraction, accompanied by a similar shift in acid phosphatase activities. Isopycnic centrifugation of mixtures of M + L fractions and brucellae permitted complete separation of acid phosphatase-bearing particles from bacteria. Similar experiments with fractions from infected animals showed that viable bacteria were found in both the acid phosphatase and free brucellae regions of the gradient. At 10 min postinfection, 52% of the recovered organisms were in the acid phosphatase region; at 30 min, 65%; at 60 min, 85%; and at 315 min, 79%. Detergent plus sonic treatment of an M + L fraction from the liver of an animal killed 50 min after infection caused most of the bacteria in the acid phosphatase region to shift to the region where free bacteria were found. These data suggested that brucellae sequestered in the liver were located primarily in the vacuolar apparatus of the cells which phagocytized them.
为评估豚鼠布鲁氏菌病的初始阶段，研究了羊种布鲁氏菌在各种组织以及从肝脏获得的亚细胞组分中的分布情况。静脉感染后50分钟，布鲁氏菌主要存在于血液和肝脏中，脾脏中也能回收大量细菌。对肝脏进行分级分离发现，大多数细菌存在于线粒体加溶酶体（M + L）组分中；然而，相当数量的细菌沉淀在细胞核（N）组分中。随着时间的推移，细菌逐渐从M + L组分转移到N组分，同时酸性磷酸酶活性也发生类似的转移。对M + L组分和布鲁氏菌的混合物进行等密度离心，可以将含酸性磷酸酶的颗粒与细菌完全分离。对感染动物的组分进行类似实验表明，在梯度的酸性磷酸酶区和游离布鲁氏菌区均发现了活细菌。感染后10分钟，回收的细菌中有52%在酸性磷酸酶区；30分钟时为65%；60分钟时为85%；315分钟时为79%。对感染后50分钟处死的动物肝脏的M + L组分进行去污剂加超声处理，导致酸性磷酸酶区的大多数细菌转移到游离细菌所在的区域。这些数据表明，肝脏中隔离的布鲁氏菌主要位于吞噬它们的细胞的液泡装置中。
