Saruta T, Cook R, Kaplan N M
J Clin Invest. 1972 Sep;51(9):2239-45. doi: 10.1172/JCI107032.
The effects of adrenocorticotropin (ACTH), angiotensins I and II, increased potassium, and decreased sodium concentrations upon steroid synthesis were examined by incubation of beef adrenal tissue slices. Angiotensin II shared with ACTH the need for calcium and an inhibition of its effect in the presence of puromycin but differed in not stimulating cyclic adenosine monophosphate (AMP). Angiotensin I was effective in steroidogenesis. The stimulation of aldosterone synthesis by increased potassium concentration was accompanied by an increased level of cyclic AMP and was inhibited in the presence of puromycin. Decreased sodium concentration stimulated aldosterone synthesis but, alone of these stimuli, simultaneously decreased corticosterone levels. It therefore appears that ACTH and potassium stimulate steroidogenesis at an early step in the biosynthetic pathway through the activation of cyclic AMP, whereas the effect of angiotensins I and II involve another mechanism and decreased sodium concentration affects a later step in steroidogenesis.
通过培养牛肉肾上腺组织切片，研究了促肾上腺皮质激素（ACTH）、血管紧张素I和II、钾离子浓度升高以及钠离子浓度降低对类固醇合成的影响。血管紧张素II与ACTH一样，需要钙离子参与，并且在嘌呤霉素存在的情况下其作用受到抑制，但不同的是它不会刺激环磷酸腺苷（AMP）的生成。血管紧张素I在类固醇生成中有效。钾离子浓度升高刺激醛固酮合成的同时伴随着环AMP水平的升高，并且在嘌呤霉素存在的情况下受到抑制。钠离子浓度降低刺激醛固酮合成，但在这些刺激因素中，它单独作用时会同时降低皮质酮水平。因此，似乎ACTH和钾离子通过激活环AMP在生物合成途径的早期阶段刺激类固醇生成，而血管紧张素I和II的作用涉及另一种机制，并且钠离子浓度降低影响类固醇生成的后期步骤。
