Mason R J, Stossel T P, Vaughan M
J Clin Invest. 1972 Sep;51(9):2399-407. doi: 10.1172/JCI107052.
Phagocytic vesicles were isolated from rabbit alveolar macrophages and guinea pig polymorphonuclear leukocytes that had ingested emulsified paraffin oil. Phospholipids and their fatty acids were determined in whole cells and in the phagocytic vesicle and pellet fractions separated from them. The cholesterol-to-phospholipid ratios in the vesicle fractions were distinctly higher than those of the respective whole cells or pellet fractions. The vesicle fractions also had higher phospholipid-to-protein ratios than did the whole cells. The phospholipids of the phagocytic vesicle fraction from macrophages contained relatively more sphingomyelin, lyso-(bis)phosphatidic acid, and phosphatidylserine and less lecithin, phosphatidylethanolamine, and phosphatidylinositol than did the whole cells or pellet fractions. The phospholipids of phagocytic vesicles from polymorphonuclear leukocytes contained significantly more phosphatidylinositol than did the pellet fractions. Lyso(bis)phosphatidic acid, which constituted 15% of the phospholipid in rabbit alveolar macrophages and 25% of that in their phagocytic vesicles, contained almost 60% oleic acid and 20% linoleic acid. This lipid was not detected in rabbit peritoneal macrophages or in rat alveolar macrophages.The polyunsaturated fatty acids of leukocyte phospholipids were chiefly linoleic, whereas in macrophages arachidonic accounted for almost 20% of the total fatty acids. The macrophages produced malondialdehyde when ingesting polystyrene beads or emulsified paraffin oil, from which it was inferred that peroxidation of endogenous lipid can occur during phagocytosis. Polymorphonuclear leukocytes in which less than 3% of phospholipid fatty acids were arachidonic did not produce malondialdehyde during phagocytosis of these inert particles, but did when ingesting an emulsion containing linolenate, thus providing evidence for peroxidation of ingested lipid. Isolated phagocytic vesicles from alveolar macrophages contained lipid peroxides and generated malondialdehyde when incubated with ADP, FeCl(3), and NADH.
从摄取了乳化石蜡油的兔肺泡巨噬细胞和豚鼠多形核白细胞中分离出吞噬小泡。测定了全细胞以及从全细胞中分离出的吞噬小泡和沉淀部分中的磷脂及其脂肪酸。小泡部分中的胆固醇与磷脂的比率明显高于各自的全细胞或沉淀部分。小泡部分的磷脂与蛋白质的比率也高于全细胞。巨噬细胞吞噬小泡部分的磷脂比全细胞或沉淀部分含有相对更多的鞘磷脂、溶血（双）磷脂酸和磷脂酰丝氨酸，而卵磷脂、磷脂酰乙醇胺和磷脂酰肌醇较少。多形核白细胞吞噬小泡的磷脂比沉淀部分含有明显更多的磷脂酰肌醇。溶血（双）磷脂酸在兔肺泡巨噬细胞的磷脂中占15%，在其吞噬小泡的磷脂中占25%，其中油酸含量近60%，亚油酸含量20%。在兔腹膜巨噬细胞或大鼠肺泡巨噬细胞中未检测到这种脂质。白细胞磷脂的多不饱和脂肪酸主要是亚油酸，而在巨噬细胞中花生四烯酸占总脂肪酸的近20%。巨噬细胞在摄取聚苯乙烯珠或乳化石蜡油时会产生丙二醛，由此推断在吞噬过程中内源性脂质可能会发生过氧化。在吞噬这些惰性颗粒时，磷脂脂肪酸中花生四烯酸含量低于3%的多形核白细胞不会产生丙二醛，但在摄取含亚麻酸酯的乳剂时会产生丙二醛，从而为摄取脂质的过氧化提供了证据。从肺泡巨噬细胞分离出的吞噬小泡含有脂质过氧化物，在与ADP、FeCl₃和NADH一起孵育时会产生丙二醛。
