Engel R R, Matsen J M, Chapman S S, Schwartz S
J Bacteriol. 1972 Dec;112(3):1310-5. doi: 10.1128/jb.112.3.1310-1315.1972.
Carbon monoxide formation from heme compounds by bacteria was investigated to study microbial hemoprotein catabolism with reference to heme degradation by mammalian tissues. Hemolytic and nonhemolytic bacteria were incubated aerobically and anaerobically with the following substrates: erythrocytes, hemoglobin, myoglobin, cytochrome c, hematin, iron hematoporphyrin, copper hematoporphyrin, protoporphyrin, and bilirubin. After 18 hr at 37 C the evolved CO was measured by gas chromatography. None of the bacteria formed CO anaerobically. Under aerobic conditions both alpha-hemolytic Streptococcus mitis and hemolytic Bacillus cereus formed CO from all of the heme compounds tested, whereas nonhemolytic Streptococcus mitis did not evolve CO from any of the substrates. The hemolytic bacteria did not produce CO when the iron of heme was either replaced by copper or removed, as in copper hematoporphyrin and in protoporphyrin, respectively.
为了参照哺乳动物组织的血红素降解来研究微生物血红素蛋白分解代谢，对细菌从血红素化合物中形成一氧化碳的情况进行了调查。将溶血性和非溶血性细菌分别与以下底物进行需氧和厌氧培养：红细胞、血红蛋白、肌红蛋白、细胞色素c、血红素、铁卟啉、铜卟啉、原卟啉和胆红素。在37℃下培养18小时后，通过气相色谱法测量产生的一氧化碳。没有一种细菌在厌氧条件下形成一氧化碳。在需氧条件下，α-溶血性缓症链球菌和溶血性蜡状芽孢杆菌能从所有测试的血红素化合物中形成一氧化碳，而非溶血性缓症链球菌不能从任何一种底物中产生一氧化碳。当血红素中的铁分别被铜取代或去除时，如在铜卟啉和原卟啉中，溶血性细菌不会产生一氧化碳。
