Spin C A, Chang R S, Mishra L, Golden H D
Appl Microbiol. 1972 Nov;24(5):735-41. doi: 10.1128/am.24.5.735-741.1972.
The relative capacity of several types of human cells and tissue to produce interferon was studied. Types of cells and tissue included were fibroblasts from embryos, foreskins, and biopsied skins; amnion cells; peripheral leukocytes; established lymphoid cell lines; established heteroploid cell lines; and chorioamniotic membrane. When Newcastle disease virus was used as the inducer, fibroblasts and amnion cells produced more interferon per 10(6) cells than leukocytes, lymphoid cells, and heteroploid cells. Only minor variations in interferon-producing capacity were observed among fibroblasts from 36 persons. Culture passage level, cell concentration, and inducer were factors that significantly affected interferon production.
研究了几种人类细胞和组织产生干扰素的相对能力。所包括的细胞和组织类型有胚胎、包皮和活检皮肤的成纤维细胞；羊膜细胞；外周血白细胞；已建立的淋巴细胞系；已建立的异倍体细胞系；以及绒毛羊膜。当用新城疫病毒作为诱导剂时，每10⁶个细胞中，成纤维细胞和羊膜细胞产生的干扰素比白细胞、淋巴细胞和异倍体细胞更多。在来自36人的成纤维细胞中，仅观察到产生干扰素能力的微小差异。培养传代水平、细胞浓度和诱导剂是显著影响干扰素产生的因素。
