Departments of Medicine, Microbiology, and Pediatrics, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Infect Immun. 1971 May;3(5):671-7. doi: 10.1128/iai.3.5.671-677.1971.
Production of interferon was studied in fibroblasts cultured from human fetal, neonatal, and maternal tissue. Human fetal and maternal cells were paired to diminish genetic variability. Fetal cells displayed an increased response to two inducers of interferon, virus and synthetic double-stranded ribopolynucleotide. Fetal cells released 300-fold more interferon than maternal cells on exposure to poly rI:rC. This enhanced capacity for interferon production was consistent in cultures developed from fetal skin obtained between the 10th and 20th gestational week. The response was relatively stable, persisting in cells cultured for 18 generations (about 14 weeks). On infection with Newcastle disease virus, fetal cells produced, on the average, 4 to 6.5 times more interferon than maternal or neonatal cells. The virus was adsorbed with equal efficiency by each type of cell. Increased production is apparently independent of the rates of overall protein synthesis, since fetal and maternal cells have very similar rates of total protein synthesis.
研究了从人胎儿、新生儿和产妇组织中培养的成纤维细胞产生干扰素的情况。将人胎儿和产妇细胞配对，以减少遗传变异性。胎儿细胞对两种干扰素诱导物（病毒和合成双链核糖多核苷酸）的反应增强。在暴露于多聚 rI：rC 时，胎儿细胞释放的干扰素比母体细胞多 300 倍。从妊娠第 10 至 20 周获得的胎儿皮肤培养的细胞中，这种增强的干扰素产生能力是一致的。反应相对稳定，在培养了 18 代（约 14 周）的细胞中仍然存在。感染新城疫病毒时，胎儿细胞产生的干扰素比母体或新生儿细胞平均多 4 至 6.5 倍。每一种细胞对病毒的吸附效率相同。增加的产量显然与总蛋白合成率无关，因为胎儿和母体细胞的总蛋白合成率非常相似。
