Laskey R A, Gurdon J B, Crawford L V
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3665-9. doi: 10.1073/pnas.69.12.3665.
RNA from encephalomyocarditis virus was injected into oocytes of Xenopus laevis. After incubation of the oocytes in [(35)S]methionine, polyacrylamide gel electrophoresis showed that a new series of polypeptides had been synthesized. They were identical in size to the polypeptides that appeared in ascites cells after infection with this virus. Electrophoretic and chromatographic analysis of the methionine-containing tryptic peptides from three of the induced polypeptides confirmed that they were virus-specific. All of the bands that appeared in ascites cells after infection also appeared in oocytes after injection of RNA from the virus. We conclude that Xenopus oocytes translate a mammalian viral mRNA faithfully and extensively, and perform normal post-translational modifications.
将脑心肌炎病毒的RNA注射到非洲爪蟾的卵母细胞中。在用[³⁵S]甲硫氨酸孵育卵母细胞后，聚丙烯酰胺凝胶电泳显示合成了一系列新的多肽。它们的大小与该病毒感染后腹水细胞中出现的多肽相同。对三种诱导多肽中含甲硫氨酸的胰蛋白酶肽进行电泳和色谱分析，证实它们是病毒特异性的。感染后腹水细胞中出现的所有条带在注射病毒RNA后的卵母细胞中也出现。我们得出结论，非洲爪蟾卵母细胞能忠实地、广泛地翻译哺乳动物病毒mRNA，并进行正常的翻译后修饰。
