Gamarnik A V, Andino R
Department of Microbiology and Immunology, University of California, San Francisco 94143-0414, USA.
EMBO J. 1996 Nov 1;15(21):5988-98.
We described a novel system to study poliovirus replication in Xenopus oocytes. Poliovirus RNA microinjected into Xenopus oocyte initiates a complete cycle of viral replication, yielding a high level of infectious viruses. Two distinct HeLa cell activities are required, one involved in initiation of translation and the other in RNA synthesis. The translation factor is a large cytoplasmic protein or complex, which is specifically used for initiation of poliovirus translation. The replication factor is required at early stages of RNA synthesis. Formation of infectious poliovirus is highly temperature dependent. At temperatures below 27 degrees C, capsid assembly appears to be impaired. The oocyte system described here could be useful in identifying and characterizing viral and cellular factors involved in virus replication.
我们描述了一种用于研究脊髓灰质炎病毒在非洲爪蟾卵母细胞中复制的新型系统。显微注射到非洲爪蟾卵母细胞中的脊髓灰质炎病毒RNA启动了完整的病毒复制周期，产生了高水平的感染性病毒。需要两种不同的HeLa细胞活性，一种参与翻译起始，另一种参与RNA合成。翻译因子是一种大的细胞质蛋白或复合物，专门用于脊髓灰质炎病毒翻译的起始。复制因子在RNA合成的早期阶段是必需的。感染性脊髓灰质炎病毒的形成高度依赖温度。在低于27摄氏度的温度下，衣壳组装似乎受损。这里描述的卵母细胞系统可能有助于鉴定和表征参与病毒复制的病毒和细胞因子。
