Solari A, Gatica M, Allende J E
Nucleic Acids Res. 1977 Jun;4(6):1873-80. doi: 10.1093/nar/4.6.1873.
Yeast transfer RNA specific for phenylalanine has been treated chemically to remove either one or two nucleotides of its 3' terminus and has been injected into Xenopus laevis oocytes to test whether this RNA can be repaired in vivo. The results obtained showed that oocytes could aminoacylate and thus repair tRNAPhe that has lost both its terminal adenosine and 3' phosphate. A similar result was obtained with tRNAPhe that had undergone two full cycles of 3' terminal nucleotide removal. The oocytes cannot aminoacylate tRNAPhe whose 3' terminal ribose has been oxidized with periodate or the derivative that retains a 3' phosphate after adenosine removal. In vitro assays show that the Xenopus ovary contains a tRNA nucleotidyl transferase with the properties similar to enzymes obtained from other sources which may be responsible for the 3' terminal repair observed in vitro.
已对酵母苯丙氨酸特异性转移RNA进行化学处理，去除其3'末端的一个或两个核苷酸，并将其注射到非洲爪蟾卵母细胞中，以测试这种RNA在体内是否能够被修复。所获得的结果表明，卵母细胞能够进行氨酰化，从而修复失去其末端腺苷和3'磷酸的苯丙氨酸tRNA。对经历了两个完整的3'末端核苷酸去除循环的苯丙氨酸tRNA也获得了类似结果。卵母细胞不能对其3'末端核糖已被高碘酸盐氧化的苯丙氨酸tRNA或去除腺苷后保留3'磷酸的衍生物进行氨酰化。体外试验表明，非洲爪蟾卵巢含有一种tRNA核苷酸转移酶，其性质与从其他来源获得的酶相似，这可能是体外观察到的3'末端修复的原因。
