Lachmann P J, Bowyer D E, Nicol P, Dawson R M, Munn E A
Immunology. 1973 Jan;24(1):135-45.
Experiments are described using the reactive lysis system on phospholipid dispersions to study the terminal phases of complement lysis. It has been found that, both on lecithin and on sphingomyelin-cholesterol liposomes, marker release and characteristic EM lesions can be found upon the sequential activity of C567 and C8 and C9 and that for both these parameters of lysis the action of C8 and C9 as well as of C567, are required. Lysis of liposomes is accompanied by the appearance of traces of the products of phospholipase activity in the supernate but it is believed that these represent the activity of contaminating enzymes and are the consequence and not the cause of lysis. However the possibility that lysis could be due to lipolytic activity cannot be totally excluded because of the negligible hydrolysis theoretically needed to form lesions.
本文描述了利用磷脂分散体上的反应性溶解系统进行的实验，以研究补体溶解的终末阶段。已发现，在卵磷脂和鞘磷脂 - 胆固醇脂质体上，C567、C8和C9依次发挥作用时，均可观察到标记物释放和特征性的电子显微镜损伤，而且对于这两个溶解参数而言，C8和C9以及C567的作用都是必需的。脂质体的溶解伴随着上清液中磷脂酶活性产物痕迹的出现，但据信这些代表了污染酶的活性，是溶解的结果而非原因。然而，由于理论上形成损伤所需的水解可忽略不计，因此不能完全排除溶解可能是由于脂解活性引起的可能性。
