大鼠肝脏和肝癌多核糖体在体外与剥离的糙面内质网的结合。是生物学现象还是人为假象？
Binding of rat liver and hepatoma polyribosomes to stripped rough endoplasmic reticulum in vitro. Biological or an artifact?
作者信息
Hochberg A A, Stratman F W, Zahlten R N, Morris H P, Lardy H A
出版信息
Biochem J. 1972 Nov;130(1):19-25. doi: 10.1042/bj1300019.
Exposed thiol groups do not appear to be related to the binding of (32)P-labelled polyribosomes to stripped rough endoplasmic reticulum in vitro. Treating stripped rough endoplasmic reticulum with GSSG did not diminish binding of polyribosomes, suggesting that binding in vitro has no correlation with the inhibition of protein synthesis in vitro reported by Kosower et al. (1971). Thiol reagents, which are known to dissociate ribosomes, did not significantly decrease binding of (32)P-labelled polyribosomes to stripped rough endoplasmic reticulum. Denaturing the protein of (32)P-labelled polyribosomes or stripped rough endoplasmic reticulum of liver or hepatoma with heat, trichloroacetic acid, or HClO(4) did not alter the binding in vitro. Therefore, the practice of measuring the binding of (32)P-labelled polyribosomes to stripped rough endoplasmic reticulum in vitro (Shires et al., 1971b) is an unsuitable indicator of biological significance in the intact cell.
暴露的巯基似乎与体外（32）P标记的多核糖体与脱脂粗面内质网的结合无关。用谷胱甘肽二硫化物（GSSG）处理脱脂粗面内质网不会减少多核糖体的结合，这表明体外结合与Kosower等人（1971年）报道的体外蛋白质合成抑制无关。已知能使核糖体解离的巯基试剂不会显著降低（32）P标记的多核糖体与脱脂粗面内质网的结合。用加热、三氯乙酸或高氯酸（HClO4）使肝脏或肝癌的（32）P标记的多核糖体或脱脂粗面内质网的蛋白质变性，不会改变体外结合。因此，体外测量（32）P标记的多核糖体与脱脂粗面内质网的结合（Shires等人，1971b）这种做法并不是完整细胞中生物学意义的合适指标。
Zotero 插件