大鼠肝脏粗面内质网膜蛋白在体外去除其结合核糖体前后的比较。
The comparison of rat liver rough endoplasmic reticulum membrane proteins before and after in vitro removal of its bound ribosomes.
作者信息
Czosnek H H, de Groot N, Hochberg A A
出版信息
Mol Biol Rep. 1975 Jul;2(2):113-8. doi: 10.1007/BF00357541.
PMID:808702
Abstract
The comparison of the proteins of rat liver rough membrane after stripping with EDTA or KCl-puromycin by two dimensional gel electrophoresis is described. By stripping the membrane with EDTA, most of the basic ribosomal proteins are still attached to the membrane; in contrast to the EDTA stripping method, treatment with KCl-puromycin removes most of the ribosomal proteins and does not remove any of the membranal proteins.
摘要
本文描述了用二维凝胶电泳对经乙二胺四乙酸（EDTA）或氯化钾-嘌呤霉素剥离后的大鼠肝脏粗面内质网蛋白质进行比较的情况。用EDTA剥离内质网时，大多数碱性核糖体蛋白仍附着在内质网上；与EDTA剥离方法相反，用氯化钾-嘌呤霉素处理可去除大多数核糖体蛋白，且不会去除任何膜蛋白。
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